This article will help you read and understand your pathology report for bullous pemphigoid.
by Katelynn Campbell MD and Allison Osmond MD FRCPC, updated December 11, 2020
Skin is made up of three layers: epidermis, dermis, and subcutaneous fat. The surface and the part you can see when you look at your skin is called the epidermis. The cells that make up the epidermis include squamous cells, basal cells, melanocytes, Merkel cells, and cells of the immune system. Squamous cells are also called keratinocytes because they are filled with a specialized protein called keratin which makes them strong.
The dermis is directly below the epidermis. The dermis is separated from the epidermis by a thin layer of tissue called the basement membrane. The dermis contains blood vessels and nerves. Below the dermis is a layer of fat called subcutaneous adipose tissue.
The squamous cells in the epidermis are held together using a specialized protein called a desmosome. Another specialized protein called a hemidesmosome is used to attach the squamous cells to the basement membrane. The strength and health of the skin depends on both the desmosomes and hemidesmosomes functioning normally.
Bullous pemphigoid is an autoimmune disease that causes fluid filled blisters. The blisters commonly occur on the skin, but they can also develop inside the mouth and on the eye.
The immune system is designed to protect our body from infections and to help it heal after an injury. An autoimmune disease is a condition that develops when the immune system inappropriately damages normal, healthy tissue in the body. The damage can be cause directly by immune cells or specialized proteins called antibodies that are made by immune cells. There are many kinds of autoimmune diseases and the symptoms depend on the types of tissues affected.
Bullous pemphigoid is caused by antibodies that stick to the hemidesmosomes at the bottom of the epidermis. When covered with antibodies, the hemidesmosomes are unable to function normally and the epidermis separates from the basement membrane. As a result, a fluid filled blister forms between the epidermis and the dermis
If your doctor suspects that you have a blistering disease such as bullous pemphigoid, they should remove two small samples of tissue in a procedure called a biopsy. The first sample should be placed in a preservative called formalin so it can be examined by a pathologist using a light microscope. The second sample should be placed in a special preservative that allows the tissue to be examined under an immunofluorescent microscope.
When the tissue sample is examined using a light microscope, bullous pemphigoid will show a fluid filled space separating the epidermis from the dermis below. Inflammatory cells may also be seen in the fluid or the surrounding tissue.
Immunofluorescence is a special test that allows pathologists to see where different types of proteins are in a tissue sample. For bullous pemphigoid, this test can be used to look for antibodies and to see where those antibodies are in the tissue.
To make the diagnosis of bullous pemphigoid, your pathologist should see antibodies stuck to the squamous cells at the bottom of the epidermis. If antibodies are found but they are not stuck to the squamous cells at the bottom of the epidermis, another diagnosis should be considered.