I-Fluorescence in situ hybridization (INHLANZI)

August 29, 2023

I-Fluorescence in situ hybridization (FISH) ukuhlolwa kwaselabhorethri okusetshenziswa ku-pathology ukuze kutholwe futhi kwenziwe kube okwasendaweni ubukhona noma ukungabi khona kokulandelana kwe-DNA ethile kuma-chromosome. Isebenza ngokusebenzisa ama-fluorescent probe abophezela lezo zingxenye ze-chromosome kuphela ezifana noma ezihambisana nophenyo. Ivame ukusetshenziswa ekuxilongeni umdlavuza kanye nama-syndromes athile ofuzo.

Isebenza kanjani i-fluorescence in situ hybridization?

1. Ukulungiswa kophenyo: I-fluorescent probe yakhelwe ukufanisa ukulandelana kwe-DNA okuthakaselayo. Lo mshini ube usubhalwa ngodayi we-fluorescent. Ama-probe ahlukene angalebula ngodayi abahlukene ukuze kutholwe okuqondiwe okuningi ngesikhathi esisodwa.
2. Ukulungiswa kwesampula: I-DNA kusampula (njengamaseli asuka kusampula yegazi, i-biopsy, noma izingxenye zethishu) i-denatured, okusho ukuthi iyashisisa ukuhlukanisa i-DNA enemicu ekabili ibe yimicu eyodwa.
3. I-Hybridization: I-probe efakwe ilebula ye-fluorescent isetshenziswa kusampula, lapho ifuna khona futhi ibophezele (ihlanganiselwe) ekulandeleni kwayo kwe-DNA okuhambisanayo ngaphakathi kwegenome.
4. Ukugeza: Ngemva kokuhlanganiswa, isampula liyagezwa ukuze kukhishwe noma iyiphi i-probe eyeqile engahlangani ku-DNA yayo eqondiwe. Lesi sinyathelo sisiza ukwehlisa isignali yangemuva futhi sithuthukise ukucaciswa kokutholwa.
5. Ukutholwa: Isampula ihlolwa ngaphansi kwesibonakhulu se-fluorescence. Ama-probe abophe ukulandelana kwe-DNA ethize azokhanya, futhi indawo yawo ingabonwa futhi ihlaziywe.

Kungani kwenziwa lokhu kuhlola?

Ikhono le-Fluorescence in situ hybridization lokuhlinzeka ngolwazi olunembile lofuzo ezingeni lamaselula kuyenza ibe ithuluzi eliyigugu ekuxilongeni nasekulawuleni umdlavuza nezifo zofuzo.

• Ukutholwa kokungajwayelekile kwe-chromosomal: I-FISH ingakwazi ukukhomba ukuguqulwa kofuzo okuthile, ukususwa, ukuphindaphinda, nokudluliselwa okuhlobene nezifo nezimo ezihlukahlukene zofuzo, okuhlanganisa nomdlavuza. Isibonelo, isetshenziswa kakhulu ukuthola i-chromosome yase-Philadelphia ku-chromosome ye-myeloid leukemia (CML), ukudluliselwa okuqondile phakathi kwama-chromosome 9 no-22.
• Ukuxilongwa komdlavuza kanye nokubikezelwa: Ngokukhomba izinguquko zofuzo eziqondile ezinhlotsheni ezithile zomdlavuza, i-FISH isiza ekuxilongeni lesi sifo, ekunqumeni ukubikezela kwaso, nokukhetha izindlela zokwelapha ezifanele. Ngokwesibonelo, isetshenziselwa ukubona HER2/neu ukwanda komdlavuza webele, okungaba nomthelela ezinqumweni zokwelashwa.
• Izifo zofuzo: I-FISH isetshenziswa ekuxilongeni izimo zofuzo, njenge-Down syndrome, ngokuthola ukuba khona kwekhophi eyengeziwe ye-chromosome 21.

Mayelana nalesi sihloko

Odokotela babhale lesi sihloko ukuze bakusize ufunde futhi uqonde umbiko wakho we-pathology. Xhumana nathi uma unemibuzo mayelana nalesi sihloko noma umbiko wakho we-pathology. Ukuze uthole isingeniso esiphelele sombiko wakho we-pathology, funda lesi sihloko.

Ezinye izinsiza eziwusizo

I-atlas ye-pathology